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An Investigation of the Antibiofilm and Antibacterial Effects of Ganoderma Lucidum and Nisin Compared with Chlorhexidine Mouthwash on Streptococcus Mutans
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F Mojarad    , MY Alikhani , F Daneshyar , D Abedi Firouzjaei * |
| 1.Department of Pediatric Dentistry, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, I.R.Iran. , abedidelaram@yahoo.com |
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Abstract: (185 Views) |
Background and Objective: Various antimicrobial compounds and solutions are used to control oral bacterial infections, especially Streptococcus mutans. Due to their increasing side effects, there is a great deal of interest in using natural substances and antimicrobial peptides to replace chemical drugs. Therefore, the present study was conducted to investigate the antibiofilm and antibacterial properties of Ganoderma lucidum and nisin in comparison with chlorhexidine mouthwash on Streptococcus mutans.
Methods: Streptococcus mutans strain PTCC 1683 was used in this laboratory experiment. Ganoderma lucidum extract and nisin solution were prepared after resuscitation of the standard strain in Brain Heart Infusion (BHI) Broth. Broth microdilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MCC) of Ganoderma lucidum extract, nisin and chlorhexidine mouthwash. The antibacterial and antibiofilm effect of the combination of Ganoderma lucidum extract and nisin was determined using the checkerboard assay.
Findings: The MIC of Ganoderma lucidum extract and nisin on the standard strain of Streptococcus mutans were reported to be 2.5 mg/ml and 25 μg/ml, respectively, and the MBC of Ganoderma lucidum extract and nisin on the standard strain were reported to be 2.5 mg/ml and 50 μg/ml, respectively. Moreover, this bacterium was sensitive to all dilutions of chlorhexidine mouthwash. The fractional inhibitory concentration index (FICI) did not show a synergistic effect due to the combined use of Ganoderma lucidum extract and nisin (FICI=2). After reducing the concentration of Ganoderma lucidum extract, nisin, and chlorhexidine mouthwash, the ability to inhibit biofilm formation on the studied strain was significantly reduced (p<0.05).
Conclusion: The results of this study indicated the antibiofilm and antibacterial effects of Ganoderma lucidum extract and nisin on Streptococcus mutans, but they are less effective compared with chlorhexidine mouthwash. |
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| Keywords: Ganoderma Lucidum, Nisin, Chlorhexidine, Streptococcus Mutans. |
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Full-Text [PDF 364 kb]
(14 Downloads)
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Type of Study: Applicable |
Subject:
Microbiology
Received: 2024/06/29 | Accepted: 2024/08/10 | Published: 2025/08/16
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